Sites that are important for their wildlife in Lancashire are identified by the Biological Heritage Sites Partnership (Lancashire County Council, Lancashire Wildlife Trust and Natural England) as Biological Heritage Sites (BHS) and two BHS fall completely within the parish of Roughlee Booth:
- Hollin Brow (1.03ha of grassland, Gr3 at SD841408, BHS No. 84SW11); and
- Bank Ends, Middle and Hollin Woods (6.99ha of woodland, Wd2 at SD843411, BHS No. 84SW12).
A further three BHS straddle the parish boundary:
- White Hough and Hugh Woods (1.83ha of woodland at SD835403, BHS No. 84SW08);
- Castor Gill (5.49ha of grassland and woodland at SD845416, BHS No. 84SW13); and
- Claude’s Clough – Admergill Water (11.66ha of grassland and woodland, centred on SD850419, BHS No. 84SE01).
A sixth BHS, Lower Blacko Water (4.01ha of grassland and woodland at SD856412, BHS No. 84SE02), touches the parish boundary for approx. 70m along the eastern edge of the parish.
These six areas are the most important places for wildlife in and adjacent to the parish, see map below for their locations.